Application

Research Sub CategoryTranscription Factors

Anti-ADAR2 Antibody, clone 2D1.1 is an antibody against ADAR2 for use in western blotting & ICC.

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected ADAR2 in HeLa cells.

Alexa Fluor^® is a registered trademark of Life Technologies™.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ADAR, also known as Nuclear pore protein 96 (NUP96) named after its molecular weight, or often in the literature as its yeast name, Nup145C, is one of them and NUP96 is made from the autocatalytic cleavage of a larger precursor protein named the Nuclear Pore Complex protein Nup98-Nup96, which also happens to be the source of NUP98 another important nuclear pore protein. NUC96 is found on both the nuclear and cytoplasmic sides of the NPC, whereas NUP98 apparently shuttles between the cytoplasmic side and the nucleoplasmic side of the NPC. Nup96 is part of a NP heptameric complex in the nuclear pore complex. Nup96 complexes with Sec13 in the middle of the heptamer complex. The function of the heptamer is to coat the curvature of the nuclear pore complex between the inner and outer nuclear membranes. Nup96 is predicted to be an alpha helical solenoid. The interaction between Nup96 and Sec13 is at the point of curvature in the heptameric complex. Interestingly, NUP96 and NUP98 also have a crucial role in the function of the immune system and are up regulated by interferons in response to viral infections. A heterozygous mutation in NUP96 has been shown to affect the expression of interferon-regulated genes and to disrupt the normal function of macrophages, and B and T lymphocytes, with no detectable gross pathological abnormalities in other organs. Moreover, the role of Nup96 in the immune response seems to be evolutionarily conserved, as NUP96 in Arabidopsis thaliana has also been shown to be required for the basal defense and resistance responses mediated by plant immune sensors.

Immunogen

GST-tagged recombinant protein corresponding to human ADAR2.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ADAR2 in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~98 kDa observed. Uniprot describes 6 isoforms due to alternative splicing at 198 kDa, 187 kDa, 98 kDa, 96 kDa, 196 kDa, and 186 kDa Uniprot also describes that the Nuclear pore complex protein Nup98-Nup96 is cleaved into a 98 kDa and a 96 kDa form. Uncharacterized band(s) may be observed in some cell lysates.